Research Use Only Human Galectin-3 ELISA Kit

For Research Use Only. Not for use in diagnostic procedures.

Reactivity Galectin-3 (human); also known as LGALS3, Galactoside binding lectin 3, Mac-2 antigen, CBP35
Sample Volume 30 microliters
Linear Measurement Range 1.4 ng/mL to 94.8 ng/mL
Kit Components
  • 96-well Ready-to-use Microtiter Plate (1 plate)
  • Assay Buffer (55 mL) (1 bottle)
  • Tetramethylbenzidine Substrate (15 mL) (1 bottle)
  • Stop Solution (0.5M sulfuric acid; 10 mL) (1 bottle)
  • Wash Buffer Concentrate (0.5M Tris buffered saline; 50 mL) (2 bottles)
  • Tracer Concentrate (Horseradish peroxidase labeled monoclonal) (1 bottle)
  • Protein Standard (Recombinant human galectin-3) (2 vials)
  • Low Quality Control (Recombinant human galectin-3 in protein matrix) (2 vials)
  • High Quality Control (Recombinant human galectin-3 in protein matrix) (2 vials)
  • Adhesive Plate Seals (2 seals)
Method Enzyme linked immunosorbent assay (ELISA) on a microtiter plate platform
Detection Limits Limit of Detection (LoD): 1.13 ng/mL
Limit of Blank (LoB): 0.86 ng/mL
Limit of Quantitation (LoQ): 1.32 ng/mL
Cross Reactivity Galectins (1, 2, 4, 7, 8, 9 and 12) and collagens (I and III) tested:
Mean % cross reactivity at or below 0.3 %
Interferences No interference with conjugated bilirubin (up to 16.8 mg/dL), unconjugated
bilirubin (up to 40.3 mg/dL), triglycerides (up to 3000 mg/dL). Hemolyzed
specimens and those with rheumatoid factor (> 50 IU/mL) and gamma
globulins (> 2.5 g/dL) should not be used.
Precision Average intra-assay CV: approximately 3.4 %
Kit Shelf Life 27 months from date of manufacture
Instrument Requirement Microtiter plate reader capable of reading at 450 nm

Ordering Information

Research Use Only Human Galectin-3 ELISA Kit
For Research Use Only. Not for use in diagnostic procedures.

Order Code: 12727
e-Mail: PurchaseOrders@corgenix.com